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EFFECT OF TELFERIA OCCIDENTALIS AND VERNONIA AMYGDALINA ON ANTIOXIDANTS AND MALONDIALDEHYDE IN HEART, PLASMA AND BRAIN IN HIGH SUCROSE DIET FED RATS

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RESEARCH INFORMATION

: EFFECT OF TELFERIA OCCIDENTALIS AND VERNONIA AMYGDALINA ON ANTIOXIDANTS AND MALONDIALDEHYDE IN HEART, PLASMA AND BRAIN IN HIGH SUCROSE DIET FED RATS
: Chapter 1 – 5
: 55 Pages
: #3, 000
: Ms Word format

This study, EFFECT OF TELFERIA OCCIDENTALIS AND VERNONIA AMYGDALINA ON ANTIOXIDANTS AND MALONDIALDEHYDE IN HEART, PLASMA AND BRAIN IN HIGH SUCROSE DIET FED RATS contains concise information that will serve as a framework or guide for your project work. The project study is well-researched for academic purposes and are usually provided in complete chapters with adequate References.

Keywords: EFFECT OF TELFERIA OCCIDENTALIS AND VERNONIA AMYGDALINA ON ANTIOXIDANTS  AND MALONDIALDEHYDE IN HEART, PLASMA AND BRAIN IN HIGH SUCROSE DIET FED RATS

RESEARCH BODY

 ABSTRACT

This present study was aimed at investigating the effect of the processing methods (Juice and Shaft) on oxidative stress induced by high sucrose diets on Catalase, Superoxide Dismutase (SOD) and Malondiadehyde (MDA) in the heart, brain and plasma. The juice and shaft extract of Telferia occidentalis (Ugwu) and Vernonia amygdalina (Ewuro) vegetables were processed and were administered alongside high sucrose. 104 albino rats (male) of wistar strain, weighing between 60-175g were bought and were divided into two groups, namely, Group I (control group), Group II (experimental group). The animals were weighed after acclimatization and were administered treatment according to their body weight. The Control group were fed with rat chow and the experimental group were fed with 40% sucrose palletized with their diet (rat chow). Each group were subdivided into four groups, Group 1: NOR (Normal Rat chow), NUJ (Normal Ugwu Juice), NUS (Normal Ugwu Shaft), NEJ (Normal Ewuro Juice), NES (Normal Ewuro Shaft) and the Group II : HSO (High Sucrose only), HUJ (High Sucrose Ugwu Juice), HUS (High Sucrose Ugwu Shaft), HEJ (High Sucrose Ewuro Juice) and HES (High Sucrose Ewuro Shaft). The result shows that high sucrose diets does not have an effect on the antioxidants as the levels of catalase in the brain, heart and plasma were within the normal range, except for High Sucrose Ewuro Shaft which excessively increased the level of catalase in the heart(P < 0.05). Also, the levels of malondiadehyde and superoxide dismutase in the brain, heart and plasma were all within the normal level, after administration of all the diets (P < 0.05).

TABLE OF CONTENTS

 CHAPTER ONE

1.0     Introduction

CHAPTER TWO

2.0     Cardiovascular disease

2.1     Sucrose

2.1.1  Properties of sucrose

2.1.2  Metabolism of sucrose

2.1.3  Sucrose consumption and health

2.2     Plants

2.2.1  Telferia occidentalis (Ugwu)

2.2.2  Vernonia amygdalina (Efo ewuro)

2.3     Oxidative stress

2.4     Antioxidants

2.4.1  Superoxide dismutase

2.4.2  Catalase

2.4.3  Malondialdehyde

CHAPTER THREE

3.0     Materials and Methods

3.1     Materials

3.2     Chemical reagents

3.3     Methods

3.3.1  Diet composition

3.3.2  Feeding and grouping of animals

3.3.3  Animal Sacrifice

3.3.4  Homogenate preparation

3.4     Biochemical analysis

3.5     Statistical Analysis

CHAPTER FOUR                                                                

4.0     Results

CHAPTER FIVE

5.0     Discussion, Conclusion and Recommendation

5.1     Discussion

5.2     Conclusion

5.3     Recommendation

          References

Appendix

Keywords:EFFECT OF TELFERIA OCCIDENTALIS AND VERNONIA AMYGDALINA ON ANTIOXIDANTS AND MALONDIALDEHYDE IN HEART, PLASMA AND BRAIN IN HIGH SUCROSE DIET FED RATS

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Keywords: EFFECT OF TELFERIA OCCIDENTALIS AND VERNONIA AMYGDALINA ON ANTIOXIDANTS  AND MALONDIALDEHYDE IN HEART, PLASMA AND BRAIN IN HIGH SUCROSE DIET FED RATS



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